Matt Loose on "Read Until" or Adaptive Sequencing

Matt Loose, Professor of Developmental and Computational Biology, Faculty of Medicine & Health Sciences, University of Nottingham

Bio and Contact Info


0:00 The DeepSeq Lab

5:10 More reference genomes needed in developmental biology

13:31 What is “read until” sequencing and its uses?

27:02 What % of sequencing do you do on Nanopore?

28:17 What are the goals of the UK coronavirus collaboration (COG-UK)?

Back before the world turned upside down, you know, all those years ago--early this February--a paper popped up on bioRxiv called, “Nanopore adaptive sequencing for mixed samples, whole exome capture and targeted panels." It’s an interesting paper.

In the paper, the authors, led by Matt Loose from the DeepSeq lab at the University of Nottingham, describe a method unique to nanopore sequencing where one can do "selective sequencing of single molecules in real time by individually reversing the voltage across specific nanopores.”

In other words, one can decide in the middle of sequencing a molecule that, nah . . . , this molecule bores me. I'll choose another.

Matt says Oxford Nanopore mentions the ability to do this back in 2014 in an aside. But he doesn’t fault us for just getting around to doing a podcast on it.

We do fault ourselves for just getting around to having Matt on the program. Hailing from the land of Robin Hood, it’s Matt Loose, Director of DeepSeq, previous record holder for sequencing the longest read, talking developmental biology, nanopore sequencing, and COG-UK, the UK’s collaborative effort to sequence the coronavirus.

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